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MBL抗體代理Human LC3 Polyclonal Antibody MBL公司

SOURCE: This antibody was purified from rabbit serum
using protein A agarose. The rabbit was immunized with
the recombinant human LC3 [MAP1LC3B （1-120 aa）].
FORMULATION: 100 ?L volume of PBS containing
50% glycerol, pH 7.2. No preservative is contained.
STORAGE: This antibody solution is stable for one year
from the date of purchase when stored at -20oC.
REACTIVITY: This antibody reacts with LC3
（MAP1LC3A, B, C） on Western blotting,
Immunoprecipitation, Immunohistochemistry,
Immunocytochemistry and Flow cytomrtry. It does not
react with GABARAP and GATE-16.
MBL抗體代理Human LC3 Polyclonal Antibody MBL公司

APPLICATIONS:
Western blotting; 1:1,000 for chemiluminescence detection
system
Immunoprecipitation; 2 ?L/300 ?L of cell extract from
1 x 107 cells
Immunohistochemistry; 1:1,000-1:2,000
Heat treatment is necessary for paraffin embedded
sections.
Microwave oven; 2 times for 10 minutes each in 10 mM
citrate buffer （pH 6.3）
Immunocytochemistry; 1:200-1:500
Flow cytometry; 1:200 （final concentration）
Detailed procedure is provided in the following
PROTOCOLS.

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